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Backbone and side–chain heteronuclear resonance assignments and hyperfine NMR shifts in horse cytochrome c

The [H26N, H33N] mutant of horse heart cytochrome c was expressed in E. coli during growth on isotopically enriched minimal media. Complete resonance assignments of both the diamagnetic reduced (spin zero) and paramagnetic oxidized (spin ½) states of the protein were obtained using standard triple r...

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Autori principali: Liu, Weixia, Rumbley, Jon, Englander, S. Walter, Wand, A. Joshua
Natura: Artigo
Lingua:Inglês
Pubblicazione: Cold Spring Harbor Laboratory Press 2003
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2324007/
https://ncbi.nlm.nih.gov/pubmed/12931009
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