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Use of Halogenated Precursors for Simultaneous DNA and RNA Detection by Means of Immunoelectron and Immunofluorescence Microscopy

We have developed a novel approach for in situ labeling and detection of nucleic acids in cultured cells. It is based on in vivo incorporation of chlorouridine (ClU) or iododeoxyuridine (IdU) into Chinese hamster ovary cells with the aim of labeling RNA and DNA, respectively. The halogenated nucleot...

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Detalhes bibliográficos
Main Authors: Vecchio, Lorella, Solimando, Liliana, Biggiogera, Marco, Fakan, Stanislav
Formato: Artigo
Idioma:Inglês
Publicado em: Histochemical Society 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2323116/
https://ncbi.nlm.nih.gov/pubmed/17938284
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1369/jhc.7A7225.2007
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