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Asparagine 285 plays a key role in transition state stabilization in rabbit muscle creatine kinase

To explore the possibility that asparagine 285 plays a key role in transition state stabilization in phosphagen kinase catalysis, the N285Q, N285D, and N285A site-directed mutants of recombinant rabbit muscle creatine kinase (rmCK) were prepared and characterized. Kinetic analysis of phosphocreatine...

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Detalhes bibliográficos
Main Authors: Borders, Charles L., MacGregor, Katherine M., Edmiston, Paul L., Gbeddy, Elikem R.K., Thomenius, Michael J., Mulligan, Guy B., Snider, Mark J.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2312435/
https://ncbi.nlm.nih.gov/pubmed/12592023
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