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Structural energetics of protein–carbohydrate interactions: Insights derived from the study of lysozyme binding to its natural saccharide inhibitors

High-sensitivity isothermal titration calorimetry was used to characterize the binding of the glycohydrolitic enzyme hen egg-white lysozyme to its natural saccharide inhibitors, chitobiose and chitrotriose. Measurements were done at a pH of 4.7, in the 15°C –45°C temperature range. Using a structura...

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Bibliografiske detaljer
Main Authors: García-Hernández, Enrique, Zubillaga, Rafael A., Chavelas-Adame, Eneas A., Vázquez-Contreras, Edgar, Rojo-Domínguez, Arturo, Costas, Miguel
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2003
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2312411/
https://ncbi.nlm.nih.gov/pubmed/12493836
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