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Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics

Accurate profiling of lipidomes relies upon the quantitative and unbiased recovery of lipid species from analyzed cells, fluids, or tissues and is usually achieved by two-phase extraction with chloroform. We demonstrated that methyl-tert-butyl ether (MTBE) extraction allows faster and cleaner lipid...

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Hlavní autoři: Matyash, Vitali, Liebisch, Gerhard, Kurzchalia, Teymuras V., Shevchenko, Andrej, Schwudke, Dominik
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Biochemistry and Molecular Biology 2008
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2311442/
https://ncbi.nlm.nih.gov/pubmed/18281723
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D700041-JLR200
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