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Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes
BACKGROUND: Recombinant protein production in Escherichia coli cells is a complex process, where among other parameters, plasmid copy number, structural and segregational stability of plasmid have an important impact on the success of productivity. It was recognised that a method for accurate and ra...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2311272/ https://ncbi.nlm.nih.gov/pubmed/18328094 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-7-6 |
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