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Rapid discrimination of Mycobacterium tuberculosis complex strains by ligation-mediated PCR fingerprint analysis.

A ligation-mediated PCR (LMPCR) method for the amplification of sequences flanking the IS6110 of the Mycobacterium tuberculosis complex has been developed. The method uses one primer specific for IS6110 and a second specific for a linker ligated to SalI-restricted genomic DNA. LMPCR is a rapid scree...

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Détails bibliographiques
Publié dans:J Clin Microbiol
Auteurs principaux: Prod'hom, G, Guilhot, C, Gutierrez, M C, Varnerot, A, Gicquel, B, Vincent, V
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1997
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC230178/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9399550/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.35.12.3331-3334.1997
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