PCR-enzyme-linked immunosorbent assay and sequencing as an alternative to serology for M-antigen typing of Streptococcus pyogenes.

A rapid PCR-enzyme-linked immunosorbent assay for identification of 10 important emm gene types of Streptococcus pyogenes was developed. The emm genotypes of a coded panel of strains of known M serotype were determined, and in 144 of 149 cases (97%) the results were congruous. Strains of types that...

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Publié dans:	J Clin Microbiol
Auteurs principaux:	Saunders, N A, Hallas, G, Gaworzewska, E T, Metherell, L, Efstratiou, A, Hookey, J V, George, R C
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1997
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC230041/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9316938/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.35.10.2689-2691.1997
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