Hepatitis C virus detection by single-round PCR specific for the terminal 3' noncoding region.

A single-round PCR method with primers specific for the 3' noncoding region (NCR) of hepatitis C virus (HCV) has been developed. Using a double RNAzol-B extraction, a high-temperature reverse-transcription step with SuperScript II reverse transcriptase, and a 40-cycle two-temperature PCR with a...

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Autors principals: Umlauft, F, Wong, D T, Oefner, P J, Underhill, P A, Cheung, R C, Wright, T L, Kolykhalov, A A, Gruenewald, K, Greenberg, H B
Format: Artigo
Idioma:Inglês
Publicat: 1996
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC229316/
https://ncbi.nlm.nih.gov/pubmed/8880519
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