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A Bayesian method for calculating real-time quantitative PCR calibration curves using absolute plasmid DNA standards
BACKGROUND: In real-time quantitative PCR studies using absolute plasmid DNA standards, a calibration curve is developed to estimate an unknown DNA concentration. However, potential differences in the amplification performance of plasmid DNA compared to genomic DNA standards are often ignored in cal...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2292693/ https://ncbi.nlm.nih.gov/pubmed/18298858 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-9-120 |
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