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P222-T Increasing the Ease and Speed of Eukaryotic Protein Expression: A Cell-Free In Vitro Translation System Based on Sf Insect Cell Extracts

We set out to develop an easy-to-use system for in vitro synthesis of recombinant eukaryotic proteins containing posttranslational modifications. A wide range of eukaryotic proteins require such modifications (e.g., phosphorylation, glycosylation, signal peptide cleavage) for correct folding or to d...

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Detalhes bibliográficos
Main Authors: Smith, J., Schaefer, F., Zacharias, A., Brinker-Krieger, N., von Groll, U., Kubick, S., Steinert, K.
Formato: Artigo
Idioma:Inglês
Publicado em: The Association of Biomolecular Resource Facilities 2007
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2292059/
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