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P83-M Characterization of Pil1p and Lsp1p Phosphorylation in S. cerevisiae using Tandem Affinity Purification and LC-MS/MS

In order to elucidate the signaling pathway of the human mPR-α protein, a membrane progesterone receptor, a strain of S. cerevisiae overexpressing this protein was generated. Initial global screens using 2D gels, a phospho-specific stain, and tandem MS/MS identified 35 proteins exhibiting alteration...

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Autors principals: Regalla, L. M., Stevens, S., Lyons, T. J.
Format: Artigo
Idioma:Inglês
Publicat: The Association of Biomolecular Resource Facilities 2007
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2291982/
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