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P221-M Autoinduction of Protein Expression for NMR Structural Studies

The development of the autoinduction process by Studier has greatly streamlined bacterial protein expression. In autoinduction media, bacterial expression systems controlled by lac promoters (such the pET system) grow uninduced to a high cell density and then spontaneously induce high-level protein...

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Detalhes bibliográficos
Main Authors: Reich, J. G., Leland, P. A., Rane, J. L., Blommel, P. G., Becker, K., Burns, B. T., Song, J., Markley, J. L., Fox, B. G.
Formato: Artigo
Idioma:Inglês
Publicado em: The Association of Biomolecular Resource Facilities 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2291852/
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