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Analysis of One-Step and Two-Step Real-Time RT-PCR Using SuperScript III

Real-time reverse transcription polymerase chain reaction (RT-PCR) is a commonly used technique to analyze gene expression. There has been little research conducted to test if SuperScript III quantitative one-step (reverse transcription carried out in the same tube as PCR) and two-step (reverse tran...

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Detalhes bibliográficos
Main Authors: Wacker, Michael J., Godard, Michael P.
Formato: Artigo
Idioma:Inglês
Publicado em: The Association of Biomolecular Resource Facilities 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2291734/
https://ncbi.nlm.nih.gov/pubmed/16461951
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