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Pitfalls of Quantitative Real-Time Reverse-Transcription Polymerase Chain Reaction

Polymerase chain reaction (PCR)-based assays can target either DNA (the genome) or RNA (the transcriptome). Targeting the genome generates robust data that are informative and, most importantly, generally applicable. This is because the information contained within the genome is context-independent;...

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Autori principali: Bustin, Stephen A, Nolan, Tania
Natura: Artigo
Lingua:Inglês
Pubblicazione: The Association of Biomolecular Resource Facilities 2004
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2291693/
https://ncbi.nlm.nih.gov/pubmed/15331581
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