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Heminested inverse PCR for IS6110 fingerprinting of Mycobacterium tuberculosis strains.

A heminested inverse PCR (HIP) for the amplification of sequences flanking the Mycobacterium tuberculosis insertion sequence IS6110 has been developed. The method depends upon primers that anneal to IS6110 at sites between its 5' end and the closest BsrFI site. The accuracy of HIP was demonstrated b...

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Detalles Bibliográficos
Publicado en:J Clin Microbiol
Principais autores: Patel, S, Wall, S, Saunders, N A
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 1996
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC229095/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8784570/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.34.7.1686-1690.1996
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