Enhanced amplification of hepatitis C virus (HCV) cDNA by PCR: detection of HCV RNA in archival sera.

A reverse transcription-PCR assay which successfully amplified hepatitis C virus RNA from poorly stored archival sera was optimized. Maximum sensitivity was achieved with Moloney murine leukemia virus RNase H- reverse transcriptase and by a single round of PCR amplification of a short (112-bp) fragm...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Beardsley, A M, Gowans, E J, Burrell, C J, Marmion, B P
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1996
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC229070/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8735126/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.34.6.1581-1582.1996
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