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GTP hydrolysis is required for vesicle fusion during nuclear envelope assembly in vitro

Nuclear envelope assembly was studied in vitro using extracts from Xenopus eggs. Nuclear-specific vesicles bound to demembranated sperm chromatin but did not fuse in the absence of cytosol. Addition of cytosol stimulated vesicle fusion, pore complex assembly, and eventual nuclear envelope growth. Ve...

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Format: Artigo
Idioma:Inglês
Publicat: The Rockefeller University Press 1992
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2289297/
https://ncbi.nlm.nih.gov/pubmed/1730756
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