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Recombinant antigen-based avidin-biotin microtiter enzyme-linked immunosorbent assay for serodiagnosis of invasive amebiasis.

An immunoscreening approach was used to isolate a strongly positive cDNA clone from an Entamoeba histolytica HK-9 cDNA expression library in the phage vector lambda ZAP-II. The 1.85-kb cDNA insert was found to be truncated and encoded the cysteine-rich, immunodominant domain of the antigenic 170-kDa...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:J Clin Microbiol
Hauptverfasser: Shenai, B R, Komalam, B L, Arvind, A S, Krishnaswamy, P R, Rao, P V
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1996
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC228901/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8815092/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.34.4.828-833.1996
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