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The PRESAT-vector: Asymmetric T-vector for high-throughput screening of soluble protein domains for structural proteomics
A rapid unidirectional method for cloning PCR-amplified cDNA fragments into virtually any fusion protein expression vector is described. The method, termed PRESAT-vector cloning, is based on a T-vector technique that does not require restriction endonuclease digestion of the PCR product. Subsequentl...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2286733/ https://ncbi.nlm.nih.gov/pubmed/14978305 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.03439004 |
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