A simple and sensitive DNA hybridization assay used for the routine diagnosis of human parvovirus B19 infection.
A dot blot hybridization assay for parvovirus B19 diagnosis was developed by using a PCR-generated probe, digoxigenin labelling, and chemiluminescence detection. Different labelling techniques and hybridization solutions were evaluated. From this analysis a protocol was devised for routine diagnosti...
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| Опубликовано в:: | J Clin Microbiol |
|---|---|
| Главные авторы: | , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
American Society for Microbiology (ASM)
1995
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC228444/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7494051/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.33.9.2473-2475.1995 |
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