Enzyme-linked immunoassay for detection of PCR-amplified DNA of legionellae in bronchoalveolar fluid.

A nonradioactive method is described that detects 10 to 100 legionellae in 1 ml of bronchoalveolar lavage fluid. DNA is purified by a proteinase K-phenol protocol or with a commercial DNA preparation kit and amplified by PCR with amplimers specific for the 16S rRNA gene of Legionella pneumophila. Th...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Jonas, D, Rosenbaum, A, Weyrich, S, Bhakdi, S
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1995
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC228139/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7542266/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.33.5.1247-1252.1995
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