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Alterations in sample preparation increase sensitivity of PCR assay for diagnosis of chancroid.

A PCR assay for the detection of Haemophilus ducreyi in clinical specimens taken from genital ulcers was developed. Although H. ducreyi, when present in such specimens, could be detected by PCR, the sensitivity of the assay was reduced by the presence of Taq polymerase inhibitors in the specimen. Th...

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Detalhes bibliográficos
Main Authors: Johnson, S R, Martin, D H, Cammarata, C, Morse, S A
Formato: Artigo
Idioma:Inglês
Publicado em: 1995
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC228097/
https://ncbi.nlm.nih.gov/pubmed/7790433
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