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Detection of Mycoplasma hyopneumoniae in nose swabs from pigs by in vitro amplification of the 16S rRNA gene.

We report the use of PCR to detect DNA from Mycoplasma hyopneumoniae, the etiological agent of enzootic porcine pneumonia. A primer set was designed for the amplification of a 649-bp fragment of the 16S rRNA gene from M hyopneumoniae. The PCR product was identified by ethidium bromide staining after...

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Détails bibliographiques
Publié dans:J Clin Microbiol
Auteurs principaux: Mattsson, J G, Bergström, K, Wallgren, P, Johansson, K E
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1995
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC228062/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7540629/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.33.4.893-897.1995
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