Colorimetric microwell plate hybridization assay for detection of amplified Mycobacterium tuberculosis DNA from sputum samples.

We developed a colorimetric microwell plate hybridization assay (CoMPHA) for the specific detection of 5'-biotinylated amplified Mycobacterium tuberculosis DNA. The optical densities of the CoMPHA corresponded to the initial amounts of purified template DNA. Here, we show that the CoMPHA is useful i...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Cho, S N, van der Vliet, G M, Park, S, Baik, S H, Kim, S K, Chong, Y, Kolk, A H, Klatser, P R, Kim, J D
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1995
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC228029/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7751391/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.33.3.752-754.1995
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