Nonradioactive PCR-enzyme-linked immunosorbent assay method for detection of human cytomegalovirus DNA.

We developed a rapid, sensitive, and specific PCR-based assay for human cytomegalovirus (HCMV). The assay includes primer and probe sequences derived from conserved HCMV nucleotide sequences and nonradioactive hybridization-confirmation. The assay detected between 10 and 100 viral genomes. All HCMV...

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Détails bibliographiques

Publié dans:	J Clin Microbiol
Auteurs principaux:	Allen, R D, Pellett, P E, Stewart, J A, Koopmans, M
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1995
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC228021/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7751384/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.33.3.725-728.1995
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