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Fast and faster: A designed variant of the B-domain of protein A folds in 3 μsec
We have introduced the mutation glycine 29 to alanine, designed to increase the rate of protein folding, into the B-domain of protein A (BdpA). From NMR lineshape analysis, we find the G29A mutation increases the folding rate constant by threefold; the folding time is 3 μsec. Although wild-type BdpA...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2280057/ https://ncbi.nlm.nih.gov/pubmed/15044721 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.03541304 |
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