New enzymes from environmental cassette arrays: Functional attributes of a phosphotransferase and an RNA-methyltransferase

By targeting gene cassettes by polymerase chain reaction (PCR) directly from environmentally derived DNA, we are able to amplify entire open reading frames (ORFs) independently of prior sequence knowledge. Approximately 10% of the mobile genes recovered by these means can be attributed to known prot...

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Main Authors: Nield, Blair S., Willows, Robert D., Torda, Andrew E., Gillings, Michael R., Holmes, Andrew J., Nevalainen, K.M. Helena, Stokes, H.W., Mabbutt, Bridget C.
格式: Artigo
語言:Inglês
出版: Cold Spring Harbor Laboratory Press 2004
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2279982/
https://ncbi.nlm.nih.gov/pubmed/15152095
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.04638704
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