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New enzymes from environmental cassette arrays: Functional attributes of a phosphotransferase and an RNA-methyltransferase
By targeting gene cassettes by polymerase chain reaction (PCR) directly from environmentally derived DNA, we are able to amplify entire open reading frames (ORFs) independently of prior sequence knowledge. Approximately 10% of the mobile genes recovered by these means can be attributed to known prot...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2279982/ https://ncbi.nlm.nih.gov/pubmed/15152095 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.04638704 |
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