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Monoclonal antibodies assisting refolding of firefly luciferase
The reactivation efficiency in the refolding of denatured luciferase in the presence and the absence of monoclonal antibodies (mAbs) has been studied. Luciferase could be partially reactivated when the protein was denatured in high concentrations of guanidium chloride (GdmCl; >4.5 M) and the refo...
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| Autors principals: | , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Cold Spring Harbor Laboratory Press
2004
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2279935/ https://ncbi.nlm.nih.gov/pubmed/15215528 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.04699904 |
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