Detection of Mycobacterium tuberculosis directly from spiked human sputum by Q-beta replicase-amplified assay.

We report on a rapid, sensitive, Q-Beta replicase-amplified nucleic acid hybridization assay for the detection of Mycobacterium tuberculosis directly from spiked human sputum. Specimens were processed by either an N-acetyl-L-cysteine-NaOH or a 2% NaOH digestion-decontamination method and then washed...

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Publié dans:	J Clin Microbiol
Auteurs principaux:	Shah, J S, Liu, J, Buxton, D, Stone, B, Nietupski, R, Olive, D M, King, W, Klinger, J D
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1995
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC227941/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7536213/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.33.2.322-328.1995
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