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Quantification of hepatitis C virus RNA by competitive amplification of RNA from denatured serum and hybridization on microtiter plates.

The direct detection of hepatitis C virus (HCV) RNA by PCR is widely used to determine the presence of circulating virions. The most relevant limit of this approach is the lack of quantitative information about the viral titer. We report a technique of competitive amplification allowing the estimati...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Principais autores: Ravaggi, A, Zonaro, A, Mazza, C, Albertini, A, Cariani, E
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1995
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC227929/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7714175/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.33.2.265-269.1995
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