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Detection and differentiation of antigenically distinct small round-structured viruses (Norwalk-like viruses) by reverse transcription-PCR and southern hybridization.

Application of reverse transcription (RT)-PCR to detect small round-structured viruses (SRSVs) from fecal specimens of patients with gastroenteritis has been insensitive because of the tremendous sequence heterogeneity between strains. We have designed two RT-PCR primer sets (G-1 and G-2) based on t...

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Bibliografiske detaljer
Udgivet i:J Clin Microbiol
Principais autores: Ando, T, Monroe, S S, Gentsch, J R, Jin, Q, Lewis, D C, Glass, R I
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1995
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC227881/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7699068/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.33.1.64-71.1995
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