A Method for Purifying Obligate Intracellular Coxiella burnetii that Employs Digitonin Lysis of Host Cells

Purification of the obligate intracellular bacterium Coxiella burnetii requires physical disruption of infected cells. Here we describe a gentle and safe digitonin lysis procedure to release C. burnetii from infected cells. The purity, yield, and infectivity of digitonin-prepped organisms are compar...

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Bibliographic Details
Main Authors: Cockrell, Diane C., Beare, Paul A., Fischer, Elizabeth R., Howe, Dale, Heinzen, Robert. A.
Format: Artigo
Language:Inglês
Published: 2008
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2276870/
https://ncbi.nlm.nih.gov/pubmed/18242746
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mimet.2007.12.015
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