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A Method for Purifying Obligate Intracellular Coxiella burnetii that Employs Digitonin Lysis of Host Cells
Purification of the obligate intracellular bacterium Coxiella burnetii requires physical disruption of infected cells. Here we describe a gentle and safe digitonin lysis procedure to release C. burnetii from infected cells. The purity, yield, and infectivity of digitonin-prepped organisms are compar...
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| Auteurs principaux: | , , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2008
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2276870/ https://ncbi.nlm.nih.gov/pubmed/18242746 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mimet.2007.12.015 |
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