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Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins

Many cellular events depend on a tightly compartmentalized distribution of H(+) ions across membrane-bound organelles. However, measurements of organelle pH in living cells have been scarce. Several mutants of the Aequorea victoria green fluorescent protein (GFP) displayed a pH-dependent absorbance...

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Auteurs principaux: Llopis, Juan, McCaffery, J. Michael, Miyawaki, Atsushi, Farquhar, Marilyn G., Tsien, Roger Y.
Format: Artigo
Langue:Inglês
Publié: The National Academy of Sciences 1998
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC22642/
https://ncbi.nlm.nih.gov/pubmed/9618493
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