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Differential Evanescence Nanometry: Live-Cell Fluorescence Measurements with 10-nm Axial Resolution on the Plasma Membrane

We present a method to resolve components within a diffraction-limited object by tracking simultaneously the average axial positions of two different sets of fluorescent molecules within it. The axial positions are then subtracted from each other to determine the separation of the two sets of fluoro...

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Detalhes bibliográficos
Main Authors: Saffarian, Saveez, Kirchhausen, Tomas
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2257884/
https://ncbi.nlm.nih.gov/pubmed/17993495
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.117234
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