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Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering
A family of genetically-encoded metabolite sensors has been constructed using bacterial periplasmic binding proteins (PBPs) linearly fused to protein fluorophores. The ligand-induced conformational change in a PBP allosterically regulates the relative distance and orientation of a fluorescence reson...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2253473/ https://ncbi.nlm.nih.gov/pubmed/16131659 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.051508105 |
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