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Bcl-XL as a fusion protein for the high-level expression of membrane-associated proteins

An Escherichia coli plasmid vector for the high-level expression of hydrophobic membrane proteins is described. The plasmid, pBCL, directs the expression of a target polypeptide fused to the C terminus of a mutant form of the anti-apoptotic Bcl-2 family protein, Bcl-XL, where the hydrophobic C termi...

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Detalhes bibliográficos
Main Authors: Thai, Khang, Choi, Jungyuen, Franzin, Carla M., Marassi, Francesca M.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2253446/
https://ncbi.nlm.nih.gov/pubmed/15741345
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.041244305
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