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Self-cleavage of fusion protein in vivo using TEV protease to yield native protein
Overproduction of proteins from cloned genes using fusion protein expression vectors in Escherichia coli and eukaryotic cells has increased the quantity of protein produced. This approach has been widely used in producing soluble recombinant proteins for structural and functional analysis. One major...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2253439/ https://ncbi.nlm.nih.gov/pubmed/15741334 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.041129605 |
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