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Chemical denaturation and elevated folding temperatures are required for wild-type activity and stability of recombinant Methanococcus jannaschii 20S proteasome

The 20S proteasome from the extreme thermophile Methanococcus jannaschii (Mj) was purified and sequenced to facilitate production of the recombinant proteasome in E. coli. The recombinant proteasome remained in solution at a purity level of 80–85% (according to SDS PAGE) following incubation of cell...

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Autors principals: Frankenberg, Rob J., Hsu, Tina S., Yakota, Hisao, Kim, Rosalind, Clark, Douglas S.
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 2001
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2253205/
https://ncbi.nlm.nih.gov/pubmed/11514679
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