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Mechanism of pressure-induced thermostabilization of proteins: Studies of glutamate dehydrogenases from the hyperthermophile Thermococcus litoralis
In this study, we investigated the effect of pressure on protein structure and stability at high temperature. Thermoinactivation experiments at 5 and 500 atm were performed using the wild-type (WT) enzyme and two single mutants (D167T and T138E) of the glutamate dehydrogenase (GDH) from the hyperthe...
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| Главные авторы: | , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Cold Spring Harbor Laboratory Press
2001
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2253192/ https://ncbi.nlm.nih.gov/pubmed/11514665 |
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