A novel sequence-specific RNA quantification method using nicking endonuclease, dual-labeled fluorescent DNA probe, and conformation-interchangeable oligo-DNA

We have developed a novel, single-step, isothermal, signal-amplified, and sequence-specific RNA quantification method (L-assay). The L-assay consists of nicking endonuclease, a dual-labeled fluorescent DNA probe (DL-probe), and conformation-interchangeable oligo-DNA (L-DNA). This signal-amplified as...

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Detalhes bibliográficos
Main Authors: Hosoda, Kazufumi, Matsuura, Tomoaki, Kita, Hiroshi, Ichihashi, Norikazu, Tsukada, Koji, Urabe, Itaru, Yomo, Tetsuya
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2008
Assuntos:
Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2248261/
https://ncbi.nlm.nih.gov/pubmed/18230763
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.761708
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