Crystallization and preliminary X-ray analysis of the complex of NADH and 3α-hydroxysteroid dehydrogenase from Pseudomonas sp. B-0831

The NAD(P)(+)-dependent enzyme 3α-hydroxysteroid dehydrogenase (3α-HSD) catalyzes the reversible interconversion of hydroxyl and oxo groups at position 3 of the steroid nucleus. The complex of NADH and 3α-HSD from Pseudomonas sp. B-0831 was crystallized by the hanging-drop vapour-diffusion method. R...

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Detalhes bibliográficos
Main Authors: Kataoka, Sachiyo, Nakamura, Shota, Ohkubo, Tadayasu, Ueda, Shigeru, Uchiyama, Susumu, Kobayashi, Yuji, Oda, Masayuki
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2006
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2243091/
https://ncbi.nlm.nih.gov/pubmed/16754984
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106016861
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