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Tris(hydroxymethyl)aminomethane induces conformational change and crystal-packing contraction of porcine pancreatic elastase

Porcine pancreatic elastase (PPE) was crystallized under new sulfate-free conditions containing 0.3 M NaCl and 50 mM tris(hydroxymethyl)aminomethane–HCl at pH 7.0. The crystal structure determined at 1.5 Å resolution had a unique conformation in four regions which contained loop portions. A chloride...

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Bibliografski detalji
Glavni autori: Kinoshita, Takayoshi, Yamaguchi, Asako, Tada, Toshiji
Format: Artigo
Jezik:Inglês
Izdano: International Union of Crystallography 2006
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2242949/
https://ncbi.nlm.nih.gov/pubmed/16820677
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106023001
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