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Overexpression, purification and crystallization of a choline-binding protein CbpI from Streptococcus pneumoniae

The choline-binding protein CbpI from Streptococcus pneumoniae is a 23.4 kDa protein with no known function. The protein has been successfully purified initially using Ni–NTA chromatography and to homogeneity using Q-Sepharose ion-exchange resin as an affinity column. CbpI was crystallized using PEG...

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Detalhes bibliográficos
Main Authors: Paterson, Neil G., Riboldi-Tunicliffe, Alan, Mitchell, Timothy J., Isaacs, Neil W.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2242943/
https://ncbi.nlm.nih.gov/pubmed/16820691
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106020616
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