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Purification, crystallization and preliminary crystallographic study of an IDS-epimerase from Agrobacterium tumefaciens BY6

The initial degradation of all stereoisomers of the complexing agent iminodisuccinate (IDS) is enabled by an epimerase in the bacterial strain Agrobacterium tumefaciens BY6. This protein was produced in Escherichia coli, purified and crystallized by the hanging-drop vapour-diffusion method. Crystals...

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Bibliografiske detaljer
Main Authors: Bäuerle, Bettina, Sandalova, Tatyana, Schneider, Gunter, Rieger, Paul-Gerhard
Format: Artigo
Sprog:Inglês
Udgivet: International Union of Crystallography 2006
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2242926/
https://ncbi.nlm.nih.gov/pubmed/16880546
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106024195
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