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Expression, purification and crystallization of a novel nonstructural protein VP9 from white spot syndrome virus

The nonstructural protein VP9 from white spot syndrome virus (WSSV) has been identified and expressed in Escherichia coli. To facilitate purification, a cleavable His(6) tag was introduced at the N-terminus. The native protein was purified and crystallized by vapour diffusion against mother liquor c...

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Detalhes bibliográficos
Main Authors: Liu, Yang, Sivaraman, J., Hew, Choy L.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2242916/
https://ncbi.nlm.nih.gov/pubmed/16880562
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106027436
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