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Variants of DsRed fluorescent protein: Development of a copper sensor
The fluorescence quenching of drFP583 (DsRed) protein by metal ions was investigated. CuSO(4) reversibly and pH dependently quenched the red emission at 583 nm of drFP583. The copper binding constant was 15 mM. Following random mutagenesis, blue- and red-shifted mutants of drFP583 were generated, an...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
Cold Spring Harbor Laboratory Press
2006
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2242387/ https://ncbi.nlm.nih.gov/pubmed/17008724 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.062239206 |
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