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Variants of DsRed fluorescent protein: Development of a copper sensor

The fluorescence quenching of drFP583 (DsRed) protein by metal ions was investigated. CuSO(4) reversibly and pH dependently quenched the red emission at 583 nm of drFP583. The copper binding constant was 15 mM. Following random mutagenesis, blue- and red-shifted mutants of drFP583 were generated, an...

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Hlavní autoři: Eli, Pharhad, Chakrabartty, Avijit
Médium: Artigo
Jazyk:Inglês
Vydáno: Cold Spring Harbor Laboratory Press 2006
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2242387/
https://ncbi.nlm.nih.gov/pubmed/17008724
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.062239206
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