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Variants of DsRed fluorescent protein: Development of a copper sensor

The fluorescence quenching of drFP583 (DsRed) protein by metal ions was investigated. CuSO(4) reversibly and pH dependently quenched the red emission at 583 nm of drFP583. The copper binding constant was 15 mM. Following random mutagenesis, blue- and red-shifted mutants of drFP583 were generated, an...

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Autors principals: Eli, Pharhad, Chakrabartty, Avijit
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 2006
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2242387/
https://ncbi.nlm.nih.gov/pubmed/17008724
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.062239206
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