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Intracellular expression profiles measured by real-time PCR tomography in the Xenopus laevis oocyte
Real-time PCR tomography is a novel, quantitative method for measuring localized RNA expression profiles within single cells. We demonstrate its usefulness by dissecting an oocyte from Xenopus laevis into slices along its animal–vegetal axis, extracting its RNA and measuring the levels of 18 selecte...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
2008
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2241880/ https://ncbi.nlm.nih.gov/pubmed/18039714 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm1024 |
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