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Relative quantification of mRNA: comparison of methods currently used for real-time PCR data analysis

BACKGROUND: Fluorescent data obtained from real-time PCR must be processed by some method of data analysis to obtain the relative quantity of target mRNA. The method chosen for data analysis can strongly influence results of the quantification. RESULTS: To compare the performance of six techniques w...

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Autori principali: Čikoš, Štefan, Bukovská, Alexandra, Koppel, Juraj
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2007
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2235892/
https://ncbi.nlm.nih.gov/pubmed/18093344
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2199-8-113
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